Protocols

Nucleic acid sequencing

PCR-free whole-genome sequencing
Tequila-7N single-stranded DNA methylation sequencing
In-house Share-seq on frozen cell samples
Targeted replication-aware fiber-seq (CHARGE-seq)
Single-cell long read nanopore epigenomic sequencing

Library design and assembly

sgRNA library for in vitro assay
sgRNA library for in vivo targeted recombination
Arms library for multiplexed CHARGE-seq

Protein purification

Tn5 transposase family
Hia5 methyltransferase family
scFv BG4 and SG4


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